The Integrated Biobank for Space Life Science (ibSLS) is a database for sharing of multi omics data, including transcriptome, metabolome, and phenotypes obtained from mouse habitat missions conducted by Japan Aerospace Exploration Agency (JAXA). The missions utilized a mouse experimental system for space flight, referred to as Mouse Habitat Unit (MHU), in order to achieve live-return of mice.
As of November 2020, five MHU missions shown below have conducted.
Project Theme Launch Vehicle MHU-1 Mouse Epigenetics SpX-9 MHU-2 Multi Omics Mice, Medical Proteomics SpX-12 MHU-3 Mouse Stress Defense SpX-14 MHU-4 Gravity Gateway Reflex, JAXA Moon-G mission (1) SpX-17 MHU-5 JAXA Moon-G mission (2) SpX-20
Construction and design of the ibSLS were conducted by JAXA and Tohoku Medical Megabank Organization (ToMMo), Tohoku University.
The MHU-1 mission was the first space mouse project with onboard 1 g control in order to study various physiological changes that occur in adapting to microgravity. Twelve wild-type C57BL/6J male mice were launched to the International Space Station (ISS) and housed onboard under microgravity (MG) or artificial earth-gravity (AG). After 35 days of habitation on the ISS, the mice splashed down in Pacific Ocean. The mice were transported to a laboratory for behavioral observations and the other analyses two days later. A ground control (GC) experiment precisely simulated the space experiment was conducted at JAXA Tsukuba in Japan. Six mice, which were individually housed in the same units as the flight experiment, were subjected to the analysis. The details were described in this paper.
The MHU-3 mission was conducted in order to study contributions of a transcription factor Nrf2 to the protection of mice against the stresses of space flight, and to the maintenance of homeostasis during the space travel. Six wild-type mice (FL-WT) and six Nrf2-KO mice (FL-KO) in the C57BL/6J background were launched. After 31 days of habitation on the International Space Station (ISS), the mice splashed down in Pacific Ocean. The mice were transported to a laboratory for behavioral observations and the other analyses two days later. A ground control (GC) experiment precisely simulated the space experiment was conducted at JAXA Tsukuba in Japan. Six WT (GC-WT) and six Nrf2-KO (GC-KO) mice, which were individually housed in the same units as the flight experiment, were subjected to the analysis. The details were described in this paper.
Data analysis & sharing
Now, transcriptome and metabolome data are available.
Total RNA was isolated from temporal bone (cochlea), mandibular bone (tooth root), spleen, liver, white adipose tissue, brown adipose tissue, cerebrum, kidney, and thymus. The total RNA samples were subjected to isolation of poly(A)-tailed RNA and library construction, except that total RNA from thymus was subjected to ribosomal RNA depletion and library construction using the similar step. The libraries were sequenced to generate more than 17 million reads per sample. The raw reads were mapped to the mouse mm10 genome using STAR software. Transcripts per million (TPM) values and the differentially expressed genes (DEGs) were obtained using RSEM and DESeq2 software, respectively.
Blood samples were collected from the tail at three time points and immediately centrifuged at 1580 x g for 10 min at 4°C. Metabolome analyses were performed using the MxP®Quant 500 Kit (Biocrates Life Sciences) according to the manufacturer’s instructions. The plasma samples (5 µL) were used for the UHPLC-MS/MS analyses (Xevo TQ-S system, Waters).
JAXA Tsukuba Space Center stores over 600 biospecimens remaining from spaceflight investigations conducted since 2016. Biospecimens are available for request first to Japanese investigators, then to those in the USA under the Japan-US Open Platform Partnership Program (JP-US OP3).
ibSLS Data Policy
- In the publication using the Data, please cite one or more of the result publications by the primary investigators of the JAXA Mouse Habitat Unit (MHU) missions. The result publications are listed along with the Data number and name on the ibSLS website.
- For all the publications using the Data, the language shall be included in the acknowledgement of the publication as follows:
This result is achieved based on the JAXA-ToMMo Integrated Biobank for Space Life Science (ibSLS).
2023-01-11ibSLS Version 3: Transcriptome data of MHU-1 mission
2022-05-30ibSLS Version 2: Mouse plasma metabolome data of MHU-3 mission and linkage the mouse data with human cohort database jMorp
2020-11-26ibSLS Version 1: Transcriptome data of MHU-3 mission
2019-02-08JAXA and ToMMo signed a cooperation agreement to the realization of a healthy and long-lived society